Se vc quer fazer que sua aplicação rode apenas uma vez, ou seja, se o (burro) usuario clicar mais de uma vez no icone, só vai abrir uma aplicação, e nao 2 ou 3.

public static Process RunningInstance()
            {
                Process current = Process.GetCurrentProcess();
                Process[] processes = Process.GetProcessesByName (current.ProcessName);

                //Loop through the running processes in with the same name
                foreach (Process process in processes)
                {
                    //Ignore the current process
                    if (process.Id != current.Id)
                    {
                        //Make sure that the process is running from the exe file.
                        if (Assembly.GetExecutingAssembly().Location.Replace("/", "\\") ==
                        current.MainModule.FileName)
                        {
                            //Return the other process instance.
                            return process;
                        }
                    }
                }

            //No other instance was found, return null.
            return null;
            }

  public static void HandleRunningInstance(Process instance)
            {
                //Make sure the window is not minimized or maximized
                ShowWindowAsync (instance.MainWindowHandle , WS_SHOWNORMAL);

                //Set the real intance to foreground window
                SetForegroundWindow (instance.MainWindowHandle);
            }

            [DllImport("User32.dll")]

            private static extern bool ShowWindowAsync(
            IntPtr hWnd, int cmdShow);
            [DllImport("User32.dll")] private static extern bool
            SetForegroundWindow(IntPtr hWnd);
            private const int WS_SHOWNORMAL = 1;

       [STAThread]
        static void Main()
        {

            //Get the running instance.
            Process instance = RunningInstance();

            if (instance == null)
            {
                //There isn't another instance, show our form.
                Application.Run (new frmLogin());
            }
            else
            {
                //There is another instance of this process.
                HandleRunningInstance(instance);
            }

        }

aplique isso a sua aplicação e terá sucesso…

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